Technical Tips

BOD Probes – Air Calibration

How to air-calibrate at 20°C.

  1. Fill a 300 ml BOD bottle with tap water and place in BOD incubator overnight.
  2. Next morning, turn on your DO meter and allow the system to warm up so that readings stop dropping (5-10 minutes).
  3. Take the extra BOD bottle out of the BOD incubator. Pour out all but 1″ of water and re-stopper. This is your calibration bottle at 20°C.
  4. Take your DO probe out of the overnight storage bottle and shake off all water droplets. Then place it in the 20°C calibration bottle.
  5. After readings have stabilized (typically 1-2 minutes), air-calibrate your system.
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